---
title: "Pairwise significance annotations"
slug: "pairwise-significance-annotations"
updated: 2026-05-28T06:13:15Z
published: 2026-05-28T06:13:15Z
canonical: "userguide.dataclassroom.com/pairwise-significance-annotations"
---

> ## Documentation Index
> Fetch the complete documentation index at: https://userguide.dataclassroom.com/llms.txt
> Use this file to discover all available pages before exploring further.

# Pairwise significance annotations

Having run a graph-driven [post-hoc test](/v1/docs/post-hoc-tests), you will have a table of statistical significance values (p-values), one for each **pair of sample groups**.

You can place these as annotations on the graph, as shown in the example below (you can also [open it on DataClassroom here](https://dataclassroom.com/en/dataseteditor/0d6f4b03-7297-4881-8094-b9354cf32e4f/graph)):

![](https://cdn.document360.io/62f8f41e-e041-42c4-b9bc-8ae1eb45d064/Images/Documentation/image-1779944553561.png)

These show that there is high significance between LowCal and Control, and also LowCal and LowFat, but not between LowFat and Control.

These can also be shown with an **asterisk notation** as below. 'ns' means "not significant":

![](https://cdn.document360.io/62f8f41e-e041-42c4-b9bc-8ae1eb45d064/Images/Documentation/image-1779944915304.png)

### How to add them

To add them:

- Run the statistical test, for example in this case ANOVA
- Run the post-hoc test (Tukey)
- Click the **Annotate graph** button

This is above the post-hoc results:

![](https://cdn.document360.io/62f8f41e-e041-42c4-b9bc-8ae1eb45d064/Images/Documentation/image-1779893664783.png)

### Configuring appearance

You can choose whether to display P values or asterisks, and also select per-pair which annotations to include on the graph (there might be many possible if you have a lot of groups). ![](https://cdn.document360.io/62f8f41e-e041-42c4-b9bc-8ae1eb45d064/Images/Documentation/image-1779945062804.png)

Click **Confirm** and they will appear on the graph.

A new control also appears beside the graph, allowing you to re-visit the configuration controls, or remove them:

![](https://cdn.document360.io/62f8f41e-e041-42c4-b9bc-8ae1eb45d064/Images/Documentation/image-1779945226344.png)

### Choosing baseline group(s)

If one of your groups is a "baseline" group, against which the other groups significance is to be judged, while significance between the other groups is less important, you can set that group as a baseline.

This means that the annotations will appear directly above the groups instead of with the "bracket" which can save space. In this example the Control group has been set as a baseline:

![](https://cdn.document360.io/62f8f41e-e041-42c4-b9bc-8ae1eb45d064/Images/Documentation/image-1779945428238.png)